Code: 17DAPSM Advanced Spectroscopy and Microscopy for Molecular Medicine
Lecturer: doc. RNDr. Vlastimil Fidler CSc. Weekly load: 2+0 Assessment: ZK
Department: 17101 Credits: 5 Semester: W,S
Description:
The course provides an overview of the most representative advanced optical spectroscopy methods with a high time resolution (ns, ps, fs) along with the techniques that combine such a time-resolution with a high spatial resolution. Also single-molecule and single-particle spectroscopy are covered as well as selected nonlinear spectroscopy methods - basic 2D techniques and 3-pulse fs photo echo. In particular, physical interpretation of such spectroscopic data is discussed together with a character of information on bio-functional molecular systems that can be acquired by applying an advanced optical spectroscopy.
The course is suitable especially for PhD students who are members of basic-research teams active in the core fields of molecular medicine such as chemistry, molecular photophysics and biophysics, physiology, pharmacology, etc.
Contents:

I.Molecular photophysics and the type of information that can be acquired by optical spectroscopy: overview.
II.Basic techniques to achieve the high resolution (spectral, time, and spatial) in optical spectroscopy methods.
III.Selected topics I: High (ns, ps, fs) time-resolution methods for biologically important molecular systems
IV.Selected topics II: Methods with a high spatial resolution and single-molecule spectroscopy.
V.Molecular-level study of the key biological processes in real time.

Recommended literature:
Fleming G. R., Kaufman L. J.: Ultrafast Spectroscopy. Oxford University Press, 2005 a 2009

Hof M., Hutterer R., Fidler V: Fluorescence Spectroscopy in Biology. Springer Verlag,
Heidelberg, 2004

Partioe navazující na vybrané příklady z:
Corkum P. at all. (Eds.): Ultrafast Phenomena XV /2007/ a XVI /2009/, Springer Verlag