Code: 17AMPFSB Fluorescence Spectroscopy in Biology and Medicine
Lecturer:  Weekly load: 2+0 Assessment: KZ
Department: 17101 Credits: 2 Semester: S
Description:
The course introduces students to fluorescence spectroscopy and microscopy from its basic physical principles, experimental techniques and working principles of common instrumentation to typical biomedical applications of fluorescence illustrated on selected studies published in literature. Apart from basic methods of fluorescence spectroscopy, new trends in fluorescence microscopy or photodynamic therapy (an example of use of fluorescence phenomena in clinical praxis) will be presented.
Contents:
1. Basic principles of optical spectroscopy, absorption of light, electronic transitions
2. Basic principles of fluorescence, Jabloński diagram, fluorescence and phosphorescence
3. Fluorescence spectra and quantum yield
4. Fluorescence lifetime and methods of its measurement
5. Quenching of fluorescence, static and dynamic quenching
6. Polarisation of fluorescence, time-resolved and steady-state fluorescence anisotropy
7. The influence of environment on fluorescence spectra and its use in study of biomolecules
8. Foerster resonance enrgy transfer and excimer fluorescence
9. Protein fluorescence, green fluorescent protein and its mutants
10. Basic principles of fluorescence microscopy
11. Resolution power of fluorescence microscopy and methods of its enhancement
12. Fluorescence correlation spectroscopy and its applications in biological research
13. Photodynamic therapy
14. Recapitulation, test
Recommended literature:
[1] Lakowicz J.R., Principles of Fluorescence Spectroscopy, 3rd Edition, Springer 2006
[2] Prosser V. et al. Experimentální metody biofyziky, Academia 1989
[3] Lecture notes available at course webpage containing links to other freely accessible web sources
Keywords:
fluorescent spectroscopy techniques, fluorescent probes, fluorescent microscopy, fluorescence correlation spectroscopy, photodynamic therapy