Code: 17AMBZMB Fundamentals of Molecular Biology
Lecturer: RNDr. Taťána Jarošíková CSc. Weekly load: 2+2 Assessment: Z,ZK
Department: 17101 Credits: 5 Semester: W
Description:
Structure and function of nucleic acids DNA and RNA. Replication, transcription, translation. Protein synthesis, prokaryotic and eukaryotic gene expression. Structure and function of proteins. Enzymes. Reproduction of cells, cell cycle, cell division. Biotechnology, hybridoma technology. Recombinant DNA vectors, restriction enzymes. Changes in genetic information, mutation. Methods of molecular biology - DNA isolation, centrifugation, electrophoresis, PCR. Flow cytometry. Genetic manipulation - genetic engineering, gene modification, gene splicing.
Contents:
1.Structure and function of DNA (the structure of the purine and pyrimidine bases, nucleotides, primary, secondary and tertiary structure of DNA, replication and DNA repair, DNA - the carrier of genetic information, gene structure, chromosome morphology, karyotype). Structure and function of RNA (purine and pyrimidine bases, ribosomal RNA, messenger RNA, transfer RNA, the ribosome structure and function)
2.The central dogma of molecular biology, replication, bacterial genome, eukaryotic genome.
3.The process of transcription, the complexity of eukaryotic genes posttranskripční adjustments
4.4th The process of translation, gene expression and its regulation.
5.Structure and function of proteins, the physico-chemical properties, functions in the organism, cell exporting proteins, immunoglobulins - structure and function
6.Enzymes, enzyme-catalyzed reactions in the cell in the body.
7.Biotechnology training of selected proteins. Hybridoma technology.
8.Recombinant DNA restriction enzymes, PCR - reaction.
9.Changes in genetic information - mutation, mutagens, tumor transformation.
10.Flow cytometry.
11.Cloning of DNA vectors.
12.Analysis of DNA. Isolation of DNA. Centrifugation. Electrophoresis.
13.Genetic engineering. Transgenic organisms - GMOs.
14.Using molecular biology and genetics in clinical medicine. Gene therapy.
Recommended literature:
Espero Publishing- Molecular biology of the cell. Edited by Bruce Alberts. 5th ed. New York, Garland Science, 2008 (anglicky), 2)Sambrook, J., Fritsch, E.F., Maniatis, T. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor Laboratory Press, 1989 3) http://www.lf2.cuni.cz/Projekty/prusa-DNA/newlook/defa12.htm
Keywords:
nucleic acids, (DNA, RNA) and proteins, replication, transcriptions, translations, gene expression, cell cycle, cell division, genetic engineering