Code: 17AMBLT Clinical Laboratory Instrumentation
Lecturer: Ing. Lenka Strnadová Weekly load: 1+1 Assessment: Z,ZK
Department: 17110 Credits: 4 Semester: W
Description:
Clinical laboratory instrumentation introduces principles of bioanalytical methods used in clinical diagnostics. Emphasis is put on optical methods (UV-VIS spectrophotometry, IR spectroscopy, AAS, AES, fluorimetry), NMR and X-ray analysis, electrochemical and electromigration methods (ion electrodes, biosensors, electrophoresis, isoelectric focusing), imunoassays and genetic methods (ELISA, PCR) as well as on chromatography and mass spectrometry. Contribution of lab automation to clinical diagnostics will be also discussed.

During the laboratory course students will be introduced into the basics of work in bioanalytical laboratory and lab data processing.
Contents:
1. Principles of method in clinical laboratory
2. Optical methods I
3. Optical methods II.
4. Elektrochemical methods I
5. Elektrochemical mthods II, Biosensors, automation in clinical laboratory
6. Elektromigration methods
7. Genetic methods
8. Imunochemical methods
9. Membrane processes
10. Chromatography I
11. Chromatography II
12. Lecture cancelled
13. Extraction and centrifugation.
14. Mass spectrometry I+II
Recommended literature:
1. Mikkelsen, S. R., Cortón, E.: Bioanalytical Chemistry. Wiley & Sons, Inc. Hoboken 2004.

2. Arneson, W., Brickell, J.: Clinical Chemistry - A Laboratory Perspective. F. A. Davis Company, Philadelphia 2007.

3. Gauglitz, G., Vo-Dinh, T.: Handbook of Spectroscopy. Wiley & Sons, Inc. Hoboken 2003.

4. Günzler, H., Williams, A.: Handbook of Analytical Techniques. Wiley & Sons, Inc. Hoboken 2001.

5. Nelson, D. L., Cox, M. M.: Lehninger principles of biochemistry. W. H. Freeman, New York 2008.

6. Voet, D., Voet, J. G.: Biochemistry. Wiley & Sons, Inc. Hoboken 2004.
Keywords:
Bioanalytics, chromatography, electrochemistry, electrophoresis, genetics, imunochemistry, lab automation, mass spectrometry, spectroscopy.